Globular Protein, X-ray Crystallography

Globular Protein, X-ray Crystallography _1265540470.bin PAGE PAGE 5 Globular protein, X-ray crystallographyGlobular protein, X-ray crystallographyStrategy to purify and characterize a globular protein from bacteriaA bacterium which is a microorganism has a storage f protein inside it. Escherichia coli are one of the bacteria which could be called as protein factory. The two important factors for purifying and characterizing the bacterium protein are the inclusion bodies and cellular metabolism. Some insoluble aggregates which are targeted by protein are called inclusion bodies. Cellular metabolism is directly influenced by the catalytic properties of protein. (Sorensen Mortensen, 2008)The amount of raw material and energy which is used for the maintenance of foreign DNA is known as metabolic burden, which is derived from the host metabolism. One of the most important factors which determine the ability of cell to produce a soluble recombinant protein is that there is no link between the metabolic burden and inclusion body formation but both of them are important.Structure showing the basepairing of 5'-GUC-3' to ...